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Image Search Results
Journal: bioRxiv
Article Title: Rejuvenated human amniotic fluid stem cells: a superior source of standardized induced mesenchymal stem cells for enhanced therapeutic applications
doi: 10.1101/2025.03.25.645040
Figure Lengend Snippet: A. Schematic representation of the differentiation of hiPSCs into iMSCs. B. Phase contrast image of hiPSCs (original magnification is x40). C. Immunofluorescence showing expression of TRA-1-60 in live hiPSCs (green), (original magnification is x40). D. Immunofluorescence showing expression of the pluripotency-associated markers NANOG (green), OCT4A (green), SOX2 (red), and KLF4 (red), nuclei were counterstained with DAPI (blue). Scale bar is 100 µm.
Article Snippet: The cells were then blocked for 30 minutes with blocking buffer (PBS supplemented with 2% bovine serum albumin and 0.1% Tween) and incubated overnight with primary antibodies at their optimal dilution, i.e. OCT4A (Santa Cruz), KLF4 (Stemgent), SOX2 (Abcam),
Techniques: Immunofluorescence, Expressing